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KMID : 0545120160260030596
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Journal of Microbiology and Biotechnology
2016 Volume.26 No. 3 p.596 ~ p.602
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Isorhamnetin Attenuates Staphylococcus aureus-Induced Lung Cell Injury by Inhibiting Alpha-Hemolysin Expression
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Jiang Lan Xiang
Li Hongen
Wang Lai Ying
Song Ze Xin
Shi Lei
Li Wen Hua
Deng Xu Ming
Wang Jian Feng
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Abstract
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Staphylococcus aureus, like other gram-positive pathogens, has evolved a large repertoire of virulence factors as a powerful weapon to subvert the host immune system, among which alpha-hemolysin (Hla), a secreted pore-forming cytotoxin, plays a preeminent role. We observed a concentration-dependent reduction in Hla production by S. aureus in the presence of sub-inhibitory concentrations of isorhamnetin, a flavonoid from the fruits of Hippophae rhamnoides L., which has little antibacterial activity. We further evaluate the effect of isorhamnetin on the transcription of the Hla-encoding gene hla and RNAIII, an effector molecule in the agr system. Isorhamnetin significantly down-regulated RNAIII expression and subsequently inhibited hla transcription. In a co-culture of S. aureus and lung cells, topical isorhamnetin treatment protected against S. aureus-induced cell injury. Isorhamnetin may represent a leading compound for the development of anti-virulence drugs against S. aureus infections.
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KEYWORD
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Staphylococcus aureus, alpha-hemolysin, isorhamnetin, anti-virulence
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